英语译文展示 2012.10.31

摘要

目的:探讨酪氨酸蛋白激酶受体EphA2表达与结直肠癌之间的关系。

方法:应用免疫组化方法检测正常结直肠组织标本和相应腺瘤和腺癌组织中EphA2蛋白的表达情况,及erphin和E-钙粘素水平。体外实验阻断E-phA2表达检测erphin和E-钙粘素水平变化和细胞活力、细胞凋亡和周期特性的改变;阻断E-cadherin表达检测EphA2水平的变化,验证EphA2蛋白与erphin和E-钙粘素之间的关系。

结果:EphA2及erphin和E-钙粘素蛋白在腺瘤和癌组织中的表达明显高于相应正常结直肠癌组织,且癌组织中的表达水平高于腺瘤组织(P<0.05)。EPHA2/erphrinA1信号通路与细胞活力、细胞凋亡和细胞周期相关。

结论:E-phA2相关蛋白的高表达与结直肠有关,EPHA2/erphrinA1信号通路与E-cadherin在结直肠癌的发生发展中可能发挥着重要作用。

研究背景

结直肠(Colorectal Cancer CRC)癌是人类常见恶性肿瘤之一,居恶性肿瘤死亡原因的第二位, 近年来其呈明显上升趋势(1),世界范围内每年约有100万的新增病例,而且在发达国家其死亡率高达33%(2)。近年来随着人民生活水平的提高,膳食结构的改变,高脂肪、低纤维素饮食致使结直肠癌的发病率呈明显上升趋势。因此,对于CRC的发病机制及其预防治疗的相关研究是一个亟待解决的重要课题。

(erythropoietin-producing hepatocellular)是Eph受体酪氨酸激酶家族(RTKS)最大的一个亚族,EPHA2是EPH受体RTKS家族中的一员,是一个分子量为130KD的跨膜蛋白,EphA,包括一个氨基酸末端胞外配体结合域、一个跨膜结构域和一个胞内酶结构域,其中胞外区由一个N端球状结构域(globular domain,Glb)、一个独特的富含半胱氨酸的和两个黏连蛋白Ⅲ型重复区( ,FN III)构成,胞内区则由具有酪氨酸激酶活性的结构域(tyrosine kinase,TK)、SAM结构域 (sterile alpha motif,SAM)和定位于C端为PDZ结构域(postsynaptic density protein,discslarge,zona occludens,PDZ)结合基序组成(3)。编码EphA2的基因定位于被称为癌症热点(cancer hot spot)染色体1p36.1上。

 

 

 

英语译文展示 2012.10.31

Abstract

Purpose: to explore the relationship between EphA2 expression of tyrosine kinase receptor and colorectal cancer.

Methods: Immunohistochemistry was used to detect the expression of EphA2 protein in normal colorectal tissue specimens and matched samples of adenoma and adenocarcinoma, and to detect erphin and E-cadherin levels. E-phA2 expression was blocked in vitro to detect the changes in erphin and E-cadherin levels and cell viability, apoptosis and changes in cell cycle. E-cadherin expression was blocked to detect changes in EphA2 levels, verifying the relationship among EphA2 protein, erphin and E-cadherin.

Results: Expressions of EphA2, erphin and E-cadherin protein in adenoma and cancer tissues were significantly higher than those in matched normal colorectal tissues. In addition, the expression level in cancer tissues was higher than that in adenoma tissues (P <0.05).

Conclusions: High expression of E-phA2 related protein is related to colorectal cancer, and EPHA2/erphrinA1 signal pathway may play an important role in the occurrence and development of E-cadherin in colorectal cancer.

Study Background

Colorectal cancer (CRC) is one of the most common human malignancies, ranking the second malignant causes of death. With a conspicuously increasing incidence in recent years (1), about one million new cases occur worldwide each year, are detected. And in developed countries (2). In recent years, with the improvement in people's living standards and changes in dietary pattern, incidence of colorectal cancer caused by has increased remarkably. Therefore, study on pathogenesis of CRC, its prevention and treatment has become an urgent issue.

 


 


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